Construction and Verification of CRISPR/Cas9 Gene Editing Vector for Cassava <i>MeSSIII</i> Gene

Design and Construction of a Cloning Vector Containing the hspX Gene of Mycobacterium tuberculosis

Background: Tuberculosis (TB) is a major cause of death worldwide. Finding an effective vaccine against TB is the best way to control it. Several vaccines against this disease have been developed but none are completely protective. The aim of this study was to design and construct a cloning vector containing the Mycobacterium tuberculosis (M. tuberculosis) heat shock protein X (hspX). Metho...

construction and evaluation of a specific nonviral vector for ex-vivo gene correction of human β-globin gene defects

Construction and cloning of a recombinant expression vector containing human Cd20 Gene for antibody therapy in Non-Hodgkin Lymphoma

ABSTRACT Introduction: Non-Hodgkin lymphoma (NHL) is a cancer that starts in lymphocytes. The main treatment for NHL is chemotherapy and radiation. Today immunotherapy is a promising therapeutic approach in the treatment of a variety cancers which is high specific unlike previous methods. Antibodies do not penetrate effectively into tumore tissues because of their large size. Whe...

Simplified method for gene targeting vector construction.

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Dual Promoter Vector Construction for Simultaneous Gene Expression Using Spliced Overlap Extension by Polymerase Chain Reaction (SOE-PCR) Technique

There are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. Bicistronic system has disadvantages e.g. compared with two-promoter system. In this paper, a simple method based on spliced overlap extension by polymerase chain reaction (SOE-PCR) technique was demonstrat...